Figure 3

PKC activation is sufficient to facilitate CSE-induced IL-8 secretion in hASMc. hASMc were stimulated with PMA (0.1 μM), in the absence or presence of CSE (5%) and GF109203X (3 μM) (Figure 3A), SC514 (50 μM) (Figure 3B) or U0126 (3 μM) (Figure 3C) for 24 hours. Supernatants were analyzed for the presence of IL-8. Data represent means ± SE of 4-6 independent experiments each performed in duplicate. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to basal; ^$$$p < 0.001 compared to PMA; ^†p < 0.05, ^††p < 0.01 and ^†††p < 0.001 compared to the absence of inhibitor (One-way ANOVA followed by Newman-Keuls multiple comparisons test). (Figure 3D) hASMc were stimulated with PMA (0.1 μM) for 1 hour. Cell lysates were analyzed for IκBα breakdown and phosphorylation of ERK1/2 by western blot. β-actin and total ERK1/2 were used as loading controls. Western blots shown are representative of 4 experiments.