Figure 8
Effects of YM976 (C, D, E, F) and theophylline (A, B) on nicotine-enhanced des-Arg9-bradykinin- (A, C, E) and bradykinin- (B, D, F) induced contractions. Tracheal segments were cultured for 4 days in absence (E, F) or presence (A-D) of nicotine (Nic, 10 μM) together with vehicle, YM976 (YM, 1 μM or 10 nM) or theophylline (TEO, 10 or 100 μM). Each data point is derived from 4-17 experiments and presented as mean ± S.E.M. Statistical analysis was performed using two-way ANOVA with Bonferroni's post-test. Nic vs Nic+TEO (A, B); Nic+vehicle vs Nic+YM (C, D); DMSO vs DMSO+YM (E, F). * P < 0.05; ** P < 0.01; *** P < 0.001.