Figure 6

Nicotine-induced effects on phosphorylated JNK (pJNK), ERK1/2 (pERK1/2) and p38 (p-p38) protein expression. Tracheal segments were cultured for 4 days in presence of vehicle (DMSO, A, C, E) or nicotine (Nic, 10 μM, B, D, F). The reference bar corresponds to 25 μm. The intensity of fluorescence was semi-quantified by Image J software (G, H, I). Epi = epithelium; SMC = smooth muscle cells; and C = cartilage. Each data point is derived from 6 experiments. Two-tailed unpaired Student's t-test with Welch's correction was preformed. Control vs Nic. ** P < 0.01; *** P < 0.001, N.S. = no significance.