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Figure 4 | Respiratory Research

Figure 4

From: Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype

Figure 4

Presence of Stro-1-, CD73-, CD105-positive cells in the lungs of OVA-treated mice. A-D. Sections from OVA-sensitized and -challenged mouse lungs (at 630× original magnification) were stained for nuclei with Hoescht 33258. These are shown as a negative grayscale image in black. AF633-conjugated anti Stro-1 is shown in blue (A), AF555-conjugated anti-CD73 is shown in red (B) and AF488-conjugated anti-CD105 is shown in green (C). In D, the merged image shows co-localization of CD73 and CD105 (yellow/orange) and Stro-1, CD73 and CD105 (white). The insets show isolated cells at higher magnification. (There is some non-specific crossreactivity with the epithelial basement membrane in the red channel.) E-H. Lung sections from OVA-treated mice were stained for nuclei with Hoechst 33258 (black), AF633-conjugated anti-Stro-1 (blue, E), AF555-conjugated anti-CD73 (red, F) and AF488-conjugated anti-α-smooth muscle actin (green, G). In H, the merged image shows co-localization of Stro-1 and CD73 (magenta), Stro-1 and α-actin (turquoise) and Stro-1, CD73 and α-actin (white). The insets show isolated cells at higher magnification. Some positive cells lie adjacent to the airway smooth muscle layer.

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