Dynamics of Cytokine Expression in blood during Acute RSV Infection. Blood CCL2, CXCL1, IFN-γ and IL-4 mRNA expression was assessed from 4-6 mice per time-point per group in 3 independent experiments from days 1 to 7 after inoculation (Fig. 4A-D). Mice were intranasally inoculated with live-RSV (solid grey bars), heat-inactivated RSV (hatched bars), or 10% EMEM (solid black bars). Values are represented as means ± SD. One-way ANOVA was used for comparisons with non-infected controls mice (*p < .001). When this test demonstrated a significant difference between groups the Tukey test to correct for multiple comparisons was used and considered significant for a p < 0.05.