Table 1 Primer sequences as used for real time RT-PCR
primer sense, 5'→3' | primer antisense, 5'→3' | |
|---|---|---|
TLR1 | AGCAAAGAAATAGATTACACATCA | TTACCTACATCATACACTCACAAT |
TLR2 | GCAAGCTGCGGAAGATAATG | CGCAGCTCTCAGATTTACCC |
TLR3 | GAATGTTTAAATCTCACTGC | AAGTGCTACTTGCAATTTAT |
TLR4 | ATGAAATGAGTTGCAGCAGA | AGCCATCGTTGTCTCCCTAA |
TLR5 | GTACAGAAACAGCAGTATTTGAG | TCTGTTGAGAGAGTTTATGAAGAA |
TLR6 | TTTACTTGGATGATGATGAATAGT | AGTTCCCCAGATGAAACATT |
TLR7 | CCATACTTCTGGCAGTGTCT | ACTAGGCAGTTGTGTTTTGC |
TLR8 | AAGAGCTCCATCCTCCAGTG | CCGTGAATCATTTTCAGTCAA |
TLR9 | GGGACAACCACCACTTCTAT | TGAGGTGAGTGTGGAGGT |
TLR10 | CAACGATAGGCGTAAATGTG | GAACCTCGAGACTCTTCATTT |
TNF-α | CTCCACCCATGTGCTCCTCA | CTCTGGCAGGGGCTCTTGAT |
IL10 | CAACAGAAGCTTCCATTCCA | AGCAGT TAGGAAGCCCCAAG |
IL6 | AATAATAATGGAAAGTGGCTATGC | AATGCCATTTATTGGTATAAAAAC |
β-Actin | TGCGTGACATTAAGGAGA AG | GTCAGGCAGCTCGTAGCTCT |