Figure 7From: Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages Activation of AM and IM by TLR9 ligands. AM or LTM were left untreated (Co) or incubated with TLR9 ligands, followed by RNA isolation and real-time PCR analysis for TNF-α (A, B), IL10 (C, D) or IL6 (E, F). A, C, E: Cells were treated either with immunostimulatory sequences (ISS 1018 phosphorothioate-modified oligonucleotide, 20 μg/ml) or genomic DNA from M. bovis BCG (20 μg/ml) for 2 h. B, D, F: DNA isolated from virulent M. tuberculosis (H37Rv) or from the attenuated H37Ra strain (20 μg/ml) was added to AM or LTM for 2 h. Data show means ± SEM of four experiments with cells derived from two different donors. *P < 0.05.Back to article page