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Figure 4 | Respiratory Research

Figure 4

From: Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages

Figure 4

Phagocytic Activity. AM and IM were cultured with fluorescent FITC-labeled microspheres for 4 h at 37°C. As a control experiment, cells were pretreated with cytochalasin D (10 μg/ml, CytD) for 1 h. Alternatively, cells were preincubated at 4°C for 1 h and incubated with microspheres for 4 h at 4°C afterwards. Experiments were performed with cells derived from at least three different donors. A, C: representative results are shown. A: Fluoresphere-associated fluorescence (marked with black bars) was detected in AM and IM using flow cytometry. B: Average of percentage of MΦ positive for fluorosphere-associated fluorescence. Data represent mean ± SEM. *P < 0.05 as compared to cells left untreated at 37°C. C: Particle uptake in AM and IM was visualized by CLSM. F-actin was stained with rhodamin-phalloidine (red), nuclei with TOTO-3 iodide (blue). Latex beads are shown in green. Co: untreated cells.

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