Skip to main content
Figure 4 | Respiratory Research

Figure 4

From: Hemoglobin α and β are ubiquitous in the human lung, decline in idiopathic pulmonary fibrosis but not in COPD

Figure 4

Modification at cysteine 105 prevents formation of Hbα complexes. (A) Schematic presentation of the spot-specific peptides, obtained by MS, and the covered protein sequence. An asterisk indicates the cysteine-containing peptide 3024.6338. MS parameters represent Aldente score, sequence coverage (SC) and the number of matched peptides (P). (B) MS spectrum representative for the Hbα monomer. (C) Gel parts corresponding to spots marked in Figure 1 revealed the presence of Hbα complexes without (-A) and with (+A) alkylation prior to separation. Homogenates of control lungs were separated by 2-DE (pH 6-11) and the total protein pattern visualized by silver staining.

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us