Figure 5

GSH and SCN levels found in the ELF are sufficient to protect against HOCl mediated toxicity in vitro. A549 cells were exposed to 750 μM HOCl (A) or 1200 μM (B) for 5 minutes and various concentrations of GSH and SCN were used in combination to determine if they were sufficient to provide protection against HOCl mediated toxicity assessed by %LDH release 24 hour after HOCl exposure. The combination of 100 μM GSH and 100 μM SCN represents basal levels found in the unexposed WT ELF, 250 μM GSH and 175 μM SCN represents WT hypertonic saline exposed levels, 50 μM GSH and 25 μM SCN represents CFTR KO unexposed basal levels, and 100 μM GSH and 25 μM SCN represents CFTR KO hypertonic saline exposed levels. Data presented as mean ± SEM, *p ≤ 0.05, ***p ≤ 0.001 compared to control.