Hypertonic saline does not affect tissue levels of GSH or SCN. Since hypertonic saline can produce a mild osmotic stress on the lung the levels GSH (A) and GSSG (B) were examined in the lung tissue. Hypertonic saline did not alter the tissue levels of either GSH or GSSG in the WT or CFTR KO mice. In addition, tissue SCN levels (C) were examined and found to be higher in the CFTR KO unexposed lung compared to the WT, but neither hypertonic nor isotonic saline showed any effect on these levels. Data presented as mean ± SEM, *p ≤ 0.05 compared between WT and CFTR KO.