Hypertonic saline exposure increases levels of antioxidant thiols in the ELF. Either WT or gut corrected CFTR KO mice were exposed to hypertonic (7%) or isotonic (0.9%) saline for 30 minutes and the antioxidant response was examined in the ELF 2 hours later. Hypertonic saline increases ELF GSH (A) in both the WT and CFTR KO mice, but the CFTR KO levels only increased back up to basal levels of the WT mice. Hypertonic saline also increases the BAL cell GSH (B) in the WT but not CFTR KO mice. Finally, hypertonic saline increases ELF SCN (C) in the WT mice but not the CFTR KO mice. Data presented as mean ± SEM, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to respective unexposed control or between WT and CFTR KO.