Experimental design of ovariectomy (OVx) and ovalbumin-(OVA) sensitization. Anesthetized rats were subjected to ovariectomy (OVx) and at the indicated day 1 were ovalbumin (OVA) sensitized. Control group consisted of rats submitted to similar manipulations excepting the ovaries removal (Sham-OVx). Sensitized rats were boosted with OVA (Day 8), and the OVA-challenge performed at day 15. The rats were euthanized at day 16 (see material and methods for more details).