Table 1 Differentially expressed proteins in the early asthmatic response
Spot* | Protein Name | Gene Symbol | Swiss-Prot/RefSeq accession No. | Theoretical Mr (kD)/pI†| Pept./% Seq.Cov‡ |
|---|---|---|---|---|---|
Down-regulated in Asthmatic rats | |||||
1 | Uteroglobin precursor | Scgb1a1 | P17559 | 10.4/4.9 | 7/25.00% |
2 | Alpha-1-antiproteinase precursor | Serpina1 | P17475 | 46.1/5.7 | 6/11.68% |
3 | Tubulin alpha-1A chain | Tuba1a | P68370 | 50.1/4.9 | 6/9.31% |
4 | Gamma-enolase | Eno2 | P07323 | 47.0/7.0 | 2/5.07% |
5 | Heat shock cognate 71 kDa protein | Hspa8 | P63018 | 70.8/5.4 | 3/4.18% |
6 | Calreticulin | Calr | P18418 | 48.0/4.3 | 49/36.06% |
7 | Phosphatidylethanolamine-binding protein 1 | Pebp1 | P31044 | 20.8/5.5 | 9/25.13% |
8 | predicted Coactosin-like 1 | Cotl1 | B0BNA5 | 15.9/5.3 | 6/27.46% |
9 | Beta-enolase | Eno3 | P15429 | 47.0/7.0 | 2/5.07% |
10 | Endoplasmic reticulum protein ERp29 precursor | Erp29 | P52555 | 28.6/6.2 | 71/48.85% |
11 | protein disulfide isomerase associated 6 | Pdia6 | Q63081 | 48.8/5.0 | 20/26.07% |
12 | Beta-enolase | Eno3 | P15429 | 47.0/7.0 | 3/5.07% |
13 | N-acetylneuraminic acid synthase | Nans | B1WC26 | 40.0/6.4 | 9/17.83% |
14 | Bifunctional purine biosynthesis protein PURH | Atic | O35567 | 64.2/6.7 | 5/6.42% |
15 | Creatine kinase B-type | Ckb | P07335 | 42.7/5.4 | 2/4.46% |
16 | Isoform 1 of Fibrinogen beta chain precursor | Fgb | P14480-1 | 54.2/7.9 | 55/45.30% |
17 | Carbonic anhydrase 2 | Ca2 | P27139 | 29.1/6.9 | 32/50.38% |
18 | 22 kDa protein | -- | -- | -- | 5/22.12% |
19 | Heat shock cognate 71 kDa protein | Hspa8 | P63018 | 70.8/5.4 | 19/18.89% |
20 | Advanced glycosylation end product-specific receptor precursor | Ager | Q63495 | 42.7/5.8 | 25/23.38% |
21 | similar to proteasome 26 S ATPase subunit 6 | Psmc6 | Q32PW9 | 45.8/7.6 | 17/27.79% |
22 | 22 kDa protein | -- | -- | -- | 7/25.00% |
23 | Cofilin-1 | Cfl1 | P45592 | 18.5/8.2 | 95/78.92% |
24 | Heat shock-related 70 kDa protein 2 | Hspa2 | P14659 | 69.6/5.5 | 6/4.27% |
25 | Phosphoglycerate mutase 1 | Pgam1 | P25113 | 28.8/6.7 | 11/16.93% |
Up-regulated in Asthmatic rats | |||||
26 | Myotrophin | Mtpn | P62775 | 12.9/5.3 | 6/25.42% |
27 | Anionic trypsin-1 precursor | Prss1 | P00762 | 26.0/4.7 | 4/8.13% |
28 | Isoform M1 of Pyruvate kinase isozymes M1/M2 | Pkm2 | P11980 | 58.0/6.6 | 40/25.52% |
29 | Protein S100-A8 | S100a8 | P50115 | 10.2/5.7 | 6/56.18% |
30 | Protein S100-A8 | S100a8 | P50115 | 10.2/5.7 | 26/41.57% |
31 | Sodium/potassium-transporting ATPase subunit alpha-1 precursor | Atp1a1 | P06685 | 11.3/5.3 | 16/13.29% |
32 | similar to destrin | LOC296197 | XP_215862 | 18.5/7.5 | 11/27.88% |
33 | Glutathione S-transferase Mu 2 | Gstm2 | P08010 | 25.7/6.9 | 18/38.99% |
34 | Tubulin alpha-1A chain | Tuba1a | P68370 | 50.1/4.9 | 11/13.53% |
35 | Rho, GDP dissociation inhibitor (GDI) beta | Arhgdib | Q5M860 | 22.9/5.0 | 4/21.00% |
36 | Serum albumin precursor | Alb | P02770 | 68.7/6.1 | 194/60.36% |
37 | Phosphoglycerate kinase 1 | Pgk1 | P16617 | 44.5/8.0 | 9/29.50% |
38 | Actin, alpha skeletal muscle | Acta1 | P68136 | 42.0/5.2 | 8/11.94% |
39 | Serum albumin precursor | Alb | P02770 | 68.7/6.1 | 7/6.58% |
40 | ATP synthase subunit alpha, mitochondrial precursor | Atp5a1 | P15999 | 59.8/6.2 | 53/22.24% |
41 | Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | P04797 | 35.8/8.1 | 5/12.31% |
42 | Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | P04797 | 35.8/8.1 | 17/29.73% |
43 | S100a11 | S100a11 | Q6B345 | 11.1/5.6 | 3/26.5% |
44 | similar to Ras suppressor protein 1 | Rsu1 | XP_001057090 | 31.5/8.5 | 4/10.83% |