Figure 3
Protein expression profiles in lung and asthmatic lung tissue. (A) Representative 2-D gel images of proteins isolated from normal control (left) and asthmatic rats (right). Total protein extracts were separated on 17-cm linear IPG strips (pH 3-10) in the first dimension followed by 12% SDS-PAGE in the second dimension and visualized by silver staining. (Left) The 25 down-regulated protein spots in asthmatic rats are marked with arrows. (Right) The 19 up-regulated protein spots in asthmatic rats are marked with arrows. The numbers correspond to the spot identification numbers listed in Table 1. The molecular weight standards and the pH range are shown at the left and the bottom of the gels, respectively. (B) Cropped 2-DE gel images of the S100A8 and RhoGDI2 protein spots. The selected area was symmetrically boxed, and the arrows indicate each protein spot or its theoretical location. (C) The expression profile of 28 most significantly changed proteins that were detected more than three times. The upper portion shows the 17 proteins down-regulated in asthmatic lung tissue, and the lower portion shows the 11 proteins that were up-regulated. Each spot volume was quantified from the intensity of the spots using PDQuest software. The bars represent the mean ± SD from three replicated 2-DE gels. The information for each altered spot is reported in Table 1.