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Figure 7 | Respiratory Research

Figure 7

From: NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigen-specific CD4+ T cell polarization

Figure 7

Pro-inflammatory cytokine production and induction of CD4 + T cell cytokine production ex vivo by NO 2 -exposed pulmonary CD11c + cells. Mice were exposed to air or 15 ppm NO2 for 1 hour followed by 30 minutes of aerosolized 3.4% ova. Forty-eight hours later, CD11c+ cells were purified from lungs via positive selection and used as antigen presenting cells co-cultured with CD4+ T cells purified from the spleen of OTII transgenic mice via positive selection. CD11c+ cells (2 × 106 cells/ml) co-cultured with T cells (1 × 106 cells/ml) were stimulated with 100 μg/ml ova323-339 and cultured for 4 days or left unstimulated in media alone. Conditioned medium were analyzed via BioPlex for pro-inflammatory mediators associated with CD11c+ cell activation (IL-1α and IL-1β) (A) and T cell polarization (IL-12p70 and IL-6) (B). Cytokines associated with T cell proliferation (IL-2) (C) as well as cytokines produced by activated Th2 cells (IL-5 and IL-13) (C, D) and Th17 cells (IL-17) (D) were measured. Data shown are mean ± SEM with 6 mice per group. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001 compared with Air Stim as determined by one-way ANOVA with Bonferroni post test.

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