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Figure 6 | Respiratory Research

Figure 6

From: NO2 inhalation induces maturation of pulmonary CD11c+ cells that promote antigen-specific CD4+ T cell polarization

Figure 6

Effects of NO 2 inhalation on antigen capture in CD11c + CD11b + lung cells in vivo . Mice were exposed to air or 15 ppm NO2 for one hour followed by oropharyngeal aspiration of 50 μg of ova-Alexa 647. Lungs were harvested 2 and 48 hours later, single cell suspensions were generated and stained with antibodies, and cells were analyzed by flow cytometry. Dead cells were excluded by FSC and SSC gating and then total lung cells were gated comparing CD11c and CD11b expression, with future analyses focusing on the CD11c+CD11b+ population (bold gate, A). The percent of CD11c+CD11b+ cells within the lung was determined (B) and uptake of the antigen ova-Alexa 647 was quantified by median fluorescence intensity (MFI) (C). NO2-exposed animals are represented by a thick line while air-exposed animals are represented by a thin line. Grey, filled histograms are naive controls (did not receive ova-Alexa 647) (C). Data shown are mean ± SEM with 4 animals per group and are representative of experiments performed twice. * denotes p < 0.05 and ** denotes p < 0.01 versus air control at the same time point by unpaired Student's t test. ^ denotes p < 0.05 and ^^ denotes p < 0.01 versus 2 hours post NO2 exposure by unpaired Student's t test.

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