Figure 1

Diphtheria toxin-mediated depletion of CD11c ⁺ cells in the lung. Eight- to fifteen-week-old female CD11c-DTR Tg⁺ and Tg^- mice were administered 4ng DT/g of body weight via i.p. injection. Twenty-four hours later, lungs were harvested, digested into single-cell suspensions, and immunostained. Total lung cells were visualized by flow cytometry (A) and CD11c⁺ cells from the parent gate were identified (B). Graphs show percent CD11c⁺ cells from the lung (C) and total number of CD11c⁺ cells in the lung, calculated based on the % CD11c⁺ cells multiplied by the number of cells in the single-cell suspension from the lungs (D). Values are mean ± SEM with 4 animals per group. ** denotes p < 0.01 by Student's t test.