Figure 5
From: Successful establishment of primary small airway cell cultures in human lung transplantation
Lineage verification of small airway epithelial cells. A & B; Gene expression of CCSP (CC-10) in small airway (SAEC) cell cultures, large airway cell cultures (LAEC) at p0 and p2 as well as large airway epithelial cell line 16HBE14o- cells as compared to the housekeeping gene 18 s. The expression of CCSP in SAEC was significantly higher than in LAEC at both p0 (p = 0.0001) and p2 (p = 0.0113) and no expression was observed in 16HBE14o-. C & D: Gene expression of Surfactant Protein-B (SP-B) in small airway (SAEC) cell cultures, large airway cell cultures (LAEC) at p0 and p2 as well as large airway epithelial cell line 16HBE14o- cells as compared to the housekeeping gene 18 s. The expression of SP-B in small airways was seen to be markedly lower than CCSP. Furthermore, the expression of SP-B in SAEC was not significantly different than in LAEC at both p0 (p = 0.4660) and p2 (p = 0.4607) (inset) and no expression was observed in 16HBE14o-.