Figure 4
From: Successful establishment of primary small airway cell cultures in human lung transplantation
Characterisation of established epithelial cell cultures. Cytospins obtained from cells cultured from large airway bronchial brushing (LAEC; Fig. 4A) and small airway brushings (SAEC; Fig. 4B) from representative lung allograft samples (at p0 and p2) were incubated with primary antibodies specific for mesenchymal (Vimentin (Vim)), endothelial (von Willebrand factor (VWF)), macrophage (CD68), dendritic (CD1a), and epithelial lineages (AE1-AE3) for 24 hours at 4°C followed by fluorescently conjugated secondary antibodies for a similar period. The slides were counterstained with 4', 6-diamidino-2-phenylindole (DAPI), which illuminates cell nuclear material (blue). Results confirmed that established cultures were not contaminated by any other cell types since and were considered pure epithelial cultures by the sole expression of all cells with the epithelial lineage marker AE1-AE3 (magnification 400×).