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Figure 2 | Respiratory Research

Figure 2

From: Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung

Figure 2

PNAd and VCAM-1 are expressed on HEVs in human BALT. (A-H): Semi-serial frozen sections of BALT (A, C, E, G; arrowheads highlight the bronchial epithelium) and PP (B, D, F, H) were stained with mAbs against PNAd (A, B), VCAM-1 (C, D) and MAdCAM-1 (E, F), or with an isotype-matched negative control mAb (G, H). (A and B) PNAd is expressed on BALT HEVs (A; arrow highlights one HEV) and on a few vessels in PP (B; arrow). (C and D) VCAM-1 is expressed on BALT HEVs (C; arrow highlights one HEV) and on one vessel in PP (D; arrow). VCAM-1 is also expressed on non-endothelial cells in BALT and PP. (E and F) MAdCAM-1 is expressed on vessels in PP (F; arrow), but not in BALT (E). (G and H) Mouse IgG1 (for anti-VCAM-1 and MAdCAM-1) and rat IgM (for anti-PNAd mAb; not shown) negative control mAbs did not stain HEVs in BALT (G) and PP (H). (A, C, E, G) The black granular material (highlighted by arrows in G) is carbon pigment in macrophages. (immunoperoxidase stains. original magnification 100×). (I-N): Semi-serial frozen sections of BALT were stained with mAbs against PNAd (green) and VCAM-1 (red) (I-K) or with anti-PNAd mAb (green) and negative control mAb (L-N) by two-color immunofluorescence. Anti-PNAd mAb gave strong staining of BALT HEVs (I, L; arrows). The anti-VCAM-1 mAb stained HEVs (J; arrow) and non-endothelial cells in BALT. PNAd+VCAM-1+ (K; arrow) and PNAd+VCAM-1- (K; arrowhead) HEVs were found in BALT. The negative control mAb for VCAM-1 did not stain BALT (M, N). (two-color immunofluorescence stains. original magnification 200×).

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