Figure 7
A) Effects of cytokines and dexamethasone (Dex) on TLR2-mediated HBD-2 expression. Epithelia were pre-treated overnight (18 hr) with cytokines (TNF-α and IFN-γ), dexamethasone, or a combination of cytokines plus dexamethasone, then exposed to Pam3CSK4 (25 μg/ml) (black), or control serum-free media (grey), for a 24-hour period. HBD-2 mRNA abundance determined by quantitative PCR. Data represent mean ± SEM from 5 donor samples. *P < 0.05, **P < 0.01. B) Effects of cytokines and dexamethasone (Dex) on TLR2-mediated CCL20 release. Primary cultures were pre-treated overnight with cytokines (TNF-α and IFN-γ), dexamethasone, or combination of cytokines plus dexamethasone, then exposed to Pam3CSK4 (25 μg/ml) (black), or control serum-free media (grey) for a period of 24 hours. Data represent mean ± SEM from 5 donor samples. *P < 0.05.