Figure 1

Stimulation of GITR enhances Th2 but not Th1 cytokine production. Co-stimulatory effect of DTA-1 on cytokine release upon CD3/CD28 activation of Th1 and Th2 lymphocytes. Th1- and Th2-polarized CD4⁺ T cells were stimulated by anti-CD3ε (1 μg/ml) and anti-CD28 (1 μg/ml) in presence of 10 μg/ml DTA-1 or control antibody (Rat IgG). After 4 days of culture, supernatants were harvested and cytokines levels (A: IL-4 in Th2 polarized cells and B: IFNγ in Th1 polarized cells) were measured by ELISA. Results are expressed as the mean of 3 independent experiments ± SEM. *: p < 0.05 as compared to cells cultured in the presence of control antibody.