Global gene expression patterns in the post-pneumonectomy lung of adult mice

Respiratory Research

Table 1 Validation of the microarray data using quantitative rt-PCR

Pathway	Gene	SABiosciences Catalog #	q-rtPCR (d1)*	Microarray (TI)*
Igf-1 signaling	Igf1	PPM03387E	1.4 (1.0 - 1.9)	1.5
	Cyr61	PPM05012A	4.0 (3.0 - 4.9)	1.6
	Igfbp2	PPM05178A	1.1 (-1.6 - 2.0)	1.5
	Igfbp3	PPM03820E	-4.0 (-8.0 - -4.0)	-1.6
Fibroblast activation	Tnfrs12a	PPM27298A	2.8 (1.8 - 4.3)	1.5
	Tnc	PPM03804E	5.0 (2.6 - 9.8)	2.2
	Col3A1	PPM04784B	1.2 (-1.1 - 1.6)	1.5
	Eln	PPM36834A	1.4 (1.1 - 2.2)	1.6

* The numbers represent the mean fold change for each gene transcript. The numbers in brackets represent the estimated range of fold change of gene expression seen in pneumonectomy animals (n = 3) compared to sham animals (n = 3), based on the standard error calculated from the pneumonectomy ΔCt values. For the microarray data, all expression values are significant with P < 0.001. TI - time-independent.

ISSN: 1465-993X