Figure 9

Cooperativity of 8-pCPT-2'- O -Me-cAMP and 6-Bnz-cAMP on bradykinin-induced IL-8 release. hTERT-airway smooth muscle cells were incubated with 50 μM 6-Bnz-cAMP alone or in combination with the indicated concentrations of 8-pCPT-2'-O-Me-cAMP (A). Alternatively, cells were stimulated with 10 μM 8-pCPT-2'-O-Me-cAMP alone or in combination with the indicated concentrations of 6-Bnz-cAMP (B). After that, 10 μM bradykinin was added for 18 hrs and IL-8 levels were measured by ELISA. Results represent mean ± SEM of separate experiments (n = 3). *P < 0.05, **P < 0.01 compared to unstimulated control.