Figure 6

Impact of Ras-like GTPases on bradykinin-induced IL-8 release. hTERT cells were treated for 24 hrs without (Control) and with 100 pg/ml of Clostridium difficile toxin B-1470 (Toxin B-1470). Then, cell morphology was assessed by phase-contrast microscopy (A). Cell number was measured on the same cells by Alamar blue as described in Material and Methods. Data represent percentage of unstimulated control (B; upper panel). In addition, IL-8 release was measured on supernatant of cells treated with 10 μM bradykinin alone or in combination with 100 μM 8-pCPT-2'-O-Me-cAMP or 500 μM 6-Bnz-cAMP in the absence (Basal) or presence of 100 pg/ml Toxin B-1470 by using ELISA (B; lower panel). Results are expressed as mean ± SEM of separate experiments (n = 4). *P < 0.05, **P < 0.01 compared to unstimulated control; ^# P < 0.05 compared to bradykinin-stimulated condition; ^§ P < 0.05 compared to basal condition.