Table 1 PCR primers
Multiplex PCR primers | Sequence | Product |
|---|---|---|
RASSF1A-F RASSF1A-R | TTAGTAAAT(C/T)GGATTAGGAGGGTTAG CCACAAAAC(A/G)AACCCC(A/G)ACTTCAAC | 325 bp (-254~+70) |
DAPK-F DAPK-R | AGGGTAGTTTAGTAATGTGTTATAG ACCCTACC(A/G)CTAC(A/G)AATTACC(A/G)AATC | 391 bp (-312~+78) |
PAX5β-F PAX5β-R | GAGTTTGTGGGTTGTTTAGTTAATGG-3' AACAAAAAATCCCAACCACCAAAACC-3' | 322 bp (-147~+174) |
tBGS Primer | Â | Â |
RASSF1A-TF RASSF1A-TR | CGACTCCTGCACTCATTAACCCTCACTAAAGAGGGT(T/C)GGATGTGGGGATTT GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGCCCAAAATCCAAACTAAAC | 337 bp (-254~+39) |
DAPK-TF DAPK-TR | CGACTCCTGCACTCATTAACCCTCACTAAAGTGGGTGTGGGG(T/C)GAGTGGGTG GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGCTCC(A/G)C(A/G)AAAAAAACAAAATC | 358 bp (-240~+50) |
PAX5β-TF PAX5β-TR | CGACTCCTGCACTCATTAACCCTCACTAAAGGTTATTTTGATTGGTTTGGTG GGCCAGTGAATTGT AATACGACTCACTATAGGGAGGCGGCTACC(A/G)AAACTAAAATAAAAC | 301 bp (-92~+141) |
| Â | Quantitative MSP primers | Â |
DAPK-qF DAPK-qR | AG(C/T)G(C/T)GGAGTTGGGAGGAGTA CAAAC(A/G)ACCAATAAAAACCCTACAAAC | 121 bp (-179~-58) |
Probe | Â | Â |
DAPK-P1m | VIC-AACGAACTAACGACGCGA-MGB | -99 - -82 |
DAPK-P1u | 6FAM-TACAAACAAACTAACAACACAA-MGB | -99 - -78 |
DAPK-P2m | VIC-CTACGCGACGCTCGC-MGB | -158 - -144 |
DAPK-P2u | 6FAM-AATTCTACACAACACTCACT-MGB | -159~-140 |