Figure 1From: Gene promoter methylation assayed in exhaled breath, with differences in smokers and lung cancer patients Tag-adapted sequencing chromatograms from exhaled breath condensate. For a portion (~250 bp) of the DAPK promoter region just 5' to the transcription initiation site (TIS), displayed for two representative subjects A and B. Top two tracings: Subject A (all CpG sites methylated, circled C's). The two top tracings are technical replicates from PCR to sequencing for this subject. Bottom tracing: Subject B (several CpG sites unmethylated, circled Ts). Detection of partial methylation at a given site is also feasible.Back to article page