Figure 6
From: The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent
GPS causes a dose-dependent change in active caspase-3 protein levels. Western blot showing cleaved caspase-3 in CCRF-CEM cells exposed to 0, 1, 2, 3, or 5 puffs GPS and analyzed 4 hr later. Lane 1) 2 μM STS-treated cells, lanes 2-6: 0-5 puffs GPS respectively. Active caspase-3, showed by an arrow, was recognized by a specific polyclonal antibody (AB3623, Chemicon), that reacts with the 17 kDa cleaved form of the enzyme. Equal loading was verified using α-tubulin as sample internal protein control. The blot is representative of three independent experiments.