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Figure 3 | Respiratory Research

Figure 3

From: The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent

Figure 3

Loss of mitochondrial membrane potential is both GPS dose-dependent and time-dependent. Δψm depolarization monitored by FACS analysis of JC-1 mitochondrial potential marker staining 2 h post-exposure (panels A and B), and 4 h post-exposure (panel C). In panels A-C representative dot plots from a single analysis are shown. Gated region R1 (red) includes cells with intact mitochondrial membranes and gated region R2 (green) depicts cells with loss of Δψm. A) Control (untreated) cells and cells treated with 2 μM staurosporine (STS; positive control) for 2 h. B) 1-5 puffs GPS-treated samples analyzed 2 h post-exposure and C) 4 h post-exposure. D) Graphic representation of mean values for R2 region data (cells with Δψm collapse) ± SD. Asterisks above bars denote p values for one way ANOVA analysis: * P < 0.0001 compared with control, ** P < 0.001 compared with control. Analyzed data derived from 4 and 3 independent experiments performed for the 2 h and 4 h time points, respectively.

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