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Figure 2 | Respiratory Research

Figure 2

From: The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent

Figure 2

GPS induces apoptotic and necrotic cell death in CCRF-CEM cells. CCRF-CEM cells were exposed to various doses of GPS (1, 3 or 5 puffs) and stained with Annexin V/PI, followed by flow cytometry analysis 2hr post-exposure. Panels A-E depict representative data. Lower left quadrants represent unstained cells and the upper left quadrants include PI-positive cells. The lower right quadrants encompass Annexin V-only positive and the upper right contain the Annexin V-FITC/PI-stained cells. A) control cells (untreated), B) 2 μM STS, C) 1 puff GPS, D) 3 puffs GPS, E) 5 puffs GPS. F) The plot represents mean values (± SD) of events for stained cells obtained from three independent experiments. * P < 0.0001 compared with control, ** P < 0.001 compared with control (one-way ANOVA).

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