Demonstration of the impact of local administration of decoy ODNs on NF-κB activation in the lungs of 92 day smoke-exposed mice. Normal saline (NS), NF-κB decoys ODNs (Decoy) or scrambled ODNs (Scr) were administered by intratracheal instillation on day 52 in smoke-exposed mice. Nuclear protein extracts were prepared from whole lung and assessed for NF-κB DNA-binding activity by electrophoretic mobility shift assay (EMSA). (A) A representative non-autoradiograph of EMSA analysis of level of NF-κB in the nuclear fraction using biotin detection. (B) A representative of the EMSA analysis of level of AP-1 in the nuclear fraction by non-autoradiograph.