Table 1 Table showing the sequences of designed and purchased rat primers and probes of MMPs investigated, the mRNA sequences used for the designs, and the conditions and optimised concentrations of each primer and probe.

Applied Biosystems | Rn01538174_m1

GATTCTGCCCAGAGACTGCTATGTC

Forward Primer

5' CCT CTA TGG ACC TCC CAC AGA A 3'

Probe

5' TAC CCA CCA AAT CTA ACT CTC TGG ACC CTG AG 3'

Reverse Primer

5' CGA AGG ACA AAG CAG AGC TAC AC 3'

Forward Primer

5' CGG CGG AGA TGC TCA CTT T 3'

Probe

5' ACA GGA AGT TCA CTC CTG AGT CCT CAC CAT C 3'

Reverse Primer

5' GCC AAG TTC ATG AGT GGC AAC 3'

Applied Biosystems | Rn00573646_m1

GAAAACTGCTGAGAATTACCTACGA

Forward Primer

5' CCG AGA CCT GAA AAC CTC CAA 3'

Probe

5' ACA CAC AGC TGG CAG AGG ATT ACC TGT ACC 3'

Reverse Primer

GCT GCC CGA GTG TAA CCA TAG 3'

Forward Primer

5' TCC TGG TGC TGC TGT GCT T 3'

Probe

5' CCG ATC TGC TCA GCA TAT CCT CTG CAT 3'

Reverse Primer

5' CTA GGT ATT GCT GAG CAA GAT CCA T 3'

Forward Primer

5' CCT GTG GAT GCA GCT TTT GAG 3'

Probe

5' CCA GAT TTG GTT CTT CCA AGG TGC TCA GTA C 3'

Reverse Primer

5' GGG CCT AGG ACT GGC TTC TC 3'

Applied Biosystems | Rn00588640_m1

TTCAAGGCACAAACCTGTTCCTTGT

Forward Primer

5' CCA GTC TCT CTA TGG TCC AGG AGA T 3'

Probe

5' AGA CCC CAA CCC TAA GCA CCC CAA AAC 3'

Reverse Primer

5' TCG GAG ACT AGT AAT GGC ATC AAG 3'

Forward Primer

5' CCG CCA TGC AAA GGT TCT A 3'

Probe

5' CGA ATC GGC CTT GCC TGT CAC TTG 3'

Reverse Primer

5' CGC CTC ATA GCC TTC ATC GT 3'

Forward Primer

5' CCA CCT TAT ACC AGC GTT ATG AGA 3'

Probe

5' CGT CGA ATC CTT TGA GCA TCT TAG TCA TCT TG 3'

Reverse Primer

5' CCG GAA ACC TGT GGC ATT T 3'

Forward Primer

5' GCT GGA CGT TGG AGG AAA GA 3'

Probe

5' TCT CCT TCC GCC TTC CCT GCA ATT AGA TAT T 3'

Reverse Primer

5' GCA CAA TAA AGT CAC AGA GGG TAA TG 3'

Forward Primer

5' GAA CGG AAG CGT GCA CAT G 3'

Probe

5' CCG ACA TCG TGA TCC GGG CC 3'

Reverse Primer

5' CCC TTC CTT CAC CAG CTT CTT 3'

Forward Primer

5' TGC AGA GGG AGA GCC TGA A 3'

Probe

5' ACT GTG GCT GCC AAA TCA CCA CTT GC 3'

Reverse Primer

5' GCC AGT CCG TCC AGA GAC A 3'