BAL MMP-9 levels in three
models of lung inflammation by zymography. A: BAL MMP-9 levels in the antigen model: rats were sensitised on days 0, 14 and 21 with ovalbumin (OVA) (100 μg, i.p.) administered with aluminium hydroxide (100 mg, i.p.) and challenged with inhaled OVA (10 g/l, 30 minutes) or saline aerosol on day 28. B: BAL MMP-9 levels in the LPS model: rats were challenged with aerosolised endotoxin free saline or aerosolised LPS (0.3 mg/ml) for 30 minutes. C: BAL MMP-9 levels in the elastase model: rats were given saline (1 ml/kg, i.t.) or PPE (120 U/kg, i.t.). All rats were sacrificed with an overdose of sodium pentobarbitone, and BAL was taken for MMP-9 analysis by zymography. Statistical significance was determined using an unpaired t test with each independent group compared to the time matched vehicle control. If the variances of the two groups were significantly different then the Mann Whitney rank sum test was used. A p-value of less than 0.05 was taken as significant and denoted with *. All the values are expressed as mean ± s.e. mean of 6 observations.