Figure 1

Antigen induced airway inflammation model. Rats were sensitised on days 0, 14 and 21 with ovalbumin (OVA) (100 μg, i.p.) administered with aluminium hydroxide (100 mg, i.p.) and challenged with inhaled OVA (10 g/l, 30 minutes) or saline aerosol on day 28. BAL samples were taken at each time point for determination of A: eosinophil levels (neutrophil and LMN levels are shown in Table 2). B: Effect of an IKK-2 inhibitor (TPCA-1) or budesonide on BAL eosinophil levels. C: Levels of p65:DNA binding in the lung tissue. Statistical significance was determined using an unpaired t test with each independent group compared to the vehicle control. If the variances of the two groups were significantly different then the Mann Whitney rank sum test was used. A p-value of less than 0.05 was taken as significant and denoted with *. All the values are expressed as mean ± s.e. mean of 6 observations.