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Figure 3 | Respiratory Research

Figure 3

From: Arginine deficiency augments inflammatory mediator production by airway epithelial cells in vitro

Figure 3

The effect of polycations poly-L-arginine and major basic protein on L- 14 C-arginine uptake and LPS-induced IL-8 production by NCI-H292 cells. Parallel cultures of NCI-H292 cells were exposed to different amounts of poly-L-arginine (A,B) or major basic protein (MBP; C,D). For arginine uptake (A, C) cells were washed with HBBS and L-14C-arginine was added for 30 min. (see Material and Methods). For IL-8 (B, D) and IL-6 (not shown) production the culture supernatants were collected after 20 h with no stimulus (white columns) or with 5 μg/ml LPS (black columns). Typical experiments are shown (triplicate samples; mean ± SEM) of 5 experiments with poly-L-arginine and 4 with MBP. *P < 0.05, **P < 0.01 and ***P < 0.001 for IL-8 production as compared to unstimulated cells; # P < 0.05, # # P < 0.01 and # # # P < 0.001 for uptake of L-14C-arginine as compared to that in the absence of poly-L-arginine or MBP.

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