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Figure 1 | Respiratory Research

Figure 1

From: Arginine deficiency augments inflammatory mediator production by airway epithelial cells in vitro

Figure 1

Enhanced production of IL-6 and IL-8 by airway epithelial cells as a function of arginine. NCI-H292 cells were plated and, after overnight culture, exposed for 24 h to fresh medium (RPMI-1640 plus L-glutamine, MEM plus L-glutamine and arginine (MEM-A+) and MEM with L-glutamine only (MEM-A-)) without and with stimuli (LPS: 5 μg/ml; TNF-α: 5 ng/ml). Typical experiment (A; IL-6 production; B: IL-8 production) is shown out of three experiments (triplicate samples; mean ± SEM, *P < 0.05; **P ≤ 0.001). Error bars may be contained within the bar. C) IL-6 production as a function of arginine concentration relative to IL-6 production in the absence of arginine (*P ≤ 0.019). D) IL-8 production by NHBE cells in MEM-A+ and MEM-A- medium (*P ≤ 0.003). E) IL-6 production by NHBE cells after 8 h. in MEM-A+ and MEM-A- medium (*P ≤ 0.012).

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