Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?

Table 1 Median (IOR) differential cell counts (%) in BALF

	Macrophages (%)	Lymphocytes (%)	Neutrophils (%)	Eosinophils (%)	Total Cells (*10⁵/ml)
OVA-1D-I	42.79(37.61)	12.50(17.85)	21.63(36.20)	23.20(18.42)	2.90(1.10)
OVA-1D-N	37.95(22.99)	6.50(2.10)	27.57(13.10)	23.20(15.17)	2.70(1.00)
OVA-3D-I	32.27(33.68)	7.17(11.89)	11.16(9.10)	40.36(18.54)^†	7.30(2.70)^††
OVA-3D-N	30.41(3.58)	8.00(16.76)	8.57(12.20)	48.71(11.70)	8.80(3.50)
Control(I)	89.13(12.54)*	7.81(17.46)	0.93(3.20)*	0.00(0.00)*	1.10(0.50)*

All mice were sensitized with OVA (10 ug, i.p) on days 0, 7 and 14. Mice in OVA-1D group were single-dose challenged on day 28. On day 29, responsiveness of mice was carried out with invasive measurements (OVA-1D-I group) or non-invasive measurements (OVA-1D-N group). Mice in OVA-3D group were three-dose challenged on day 28, 29, 30. On day 31, responsiveness of mice was carried out by invasive (OVA-3D-I group) or Penh measurements (OVA-3D-N group). The mice in Control (I) group were sensitized and challenged with normal saline.
*P ≤ 0.01 for Control group versus OVA immunized group, Kruskal-Wallis test.
^† P < 0.05 for OVA-3D-I group versus OVA-1D-I group, Kruskal-Wallis test.
^†† P ≤ 0.001 for OVA-3D-I group versus OVA-1D group (single-dose OVA challenge), Kruskal-Wallis test.

ISSN: 1465-993X