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Figure 1 | Respiratory Research

Figure 1

From: Does unrestrained single-chamber plethysmography provide a valid assessment of airway responsiveness in allergic BALB/c mice?

Figure 1

Protocol for ovalbumin (OVA) intraperitoneal (i.p.) sensitization and subsequent OVA intranasal (i.n.) challenge. Mice were sensitized by an intraperitoneal injection of 10 μg OVA on days 0, 7 and 14, followed by daily intranasal challenges with 0.2% OVA. OVA-1D-N was challenged on day 28 and airway responsiveness was carried out on day 29 by Penh measurements. OVA-1D-I were challenged on day 28 and airway responsiveness was carried out on day 29 by invasive methods. OVA-3D-N were challenged on days 28, 29, 30 and airway responsiveness was carried out on day 31 by Penh measurements. OVA-3D-I were challenged on days 28, 29, 30 and airway responsiveness was carried out on day 31 by invasive methods.

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