Figure 2
From: Oxidative stress augments toll-like receptor 8 mediated neutrophilic responses in healthy subjects
Effect of H 2 O 2 on the R848-induced cytokine release from human PMNs, and effect of N-acethyl- L -cysteine on the potentiation of cytokine release by H 2 O 2 . (A) PMNs were incubated with various concentrations of H2O2 for 30 min, and then treated with R848 for 24 hrs. Media were assayed for IL-8 by ELISA. (B) Various concentrations of R848 were added to PMNs in the presence or absence of 50 μM H2O2. After 24 hrs, IL-8 levels in media were measured by ELISA. Dose-response curve of IL-8 release from PMNs was plotted against the R848 concentration. (C) Ten mM N-acethyl-L-cysteine (NAC) was added 10 min before H2O2 or vehicle treatment, then the PMNs were cultured for 24 hrs in the presence or absence of R848. (D-F) Effects of H2O2 on TNF-α (D), IL-6 (E) and IL-1β (F) release from the R848-treated PMNs were assessed by Cytokine-Beads Array. All values are mean values ± SEM of three to five separate experiments. **p < 0.01, compared with the values of vehicle-pretreated 1 μM R848-treated group; ××p < 0.01, compared with the values of control; ≠≠p < 0.01, compared with the values of vehicle treated group; +p < 0.05, ++p < 0.01, compared with the values of 50 μM H2O2-pretreated and vehicle-treated group.