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Figure 4 | Respiratory Research

Figure 4

From: Cigarette smoke attenuates the production of cytokines by human plasmacytoid dendritic cells and enhances the release of IL-8 in response to TLR-9 stimulation

Figure 4

CSE inhibits Akt activation induced by CpG-C by human pDCs. pDCs (106cells/ml) were cultured for 90 min (A) without stimulant as control, (B) stimulated with CpG-C (1 μM), (C) pretreatment for 30 with LY294002 (10 μM) and then stimulated with CpG-C (1 μM), (D) stimulated with CSE (1.5%) and (E) in combination CSE (1.5%) and CpG-C (1 μM). Following fixation and permeabalization, cells were stained with Alexa Fluor 647 phospho anti-human Akt or Alexa 647 isotype control antibodies, as specified in the "Materials and methods". A representative of three independent experiments is shown. 3,000 events were counted in the pDC gate.

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Respiratory Research

ISSN: 1465-993X

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