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Figure 3 | Respiratory Research

Figure 3

From: Cigarette smoke attenuates the production of cytokines by human plasmacytoid dendritic cells and enhances the release of IL-8 in response to TLR-9 stimulation

Figure 3

CSE inhibits TLR-induced mRNA expression and protein production of IFN-α by pDC. pDCs (106cells/ml) were stimulated with CpG-A (2 μM), CSE (1.5%) or in combination for 5 h, then lyzed, followed by RNA extraction and real time PCR analysis. IFN-α mRNA expression is normalized to housekeeping gene (β2-microglobulin) expression, and shown as fold induction over expression of non-stimulated control cells (A). pDCs (1 × 106/ml) were stimulated with CpG-A (2 μM), CSE (1.5%) or incombination and CSE (1.5%) for 8 h (B). IFN-α concentration of culture supernatants were determined by ELISA (mean ± SEM of 3 independent experiments; ***p < 0.001).

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Respiratory Research

ISSN: 1465-993X

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