Figure 5

TLR3 KO mice are partially protected from poly(I:C)-induced inflammatory cell influx in the airways. Mice were administered PBS or 100 μg poly(I:C) I.N. every 24 h for three days. 24 hours after the last poly(I:C) administration, mice were euthanized and the lungs were lavaged. The total number of cells (5A), neutrophils (5B) and mononuclear cells(5C) were measured in the BAL. Data are the mean ± SEM of 6 mice. Treatment groups (PBS or 100 μg poly(I:C)) and mouse types were compared using 2-way ANOVA, including an interaction term. *p < 0.05, **p < 0.01 compared to PBS-treated mice. When comparing the impact of poly(I:C) treatment on cell populations in the lavage, there was a significantly larger increase in the response of wild type mice than knockout mice, with respect to total cells and mononuclear cells alone, **p < 0.01 in each case. Similar trends were observed in neutrophils alone but failed to reach statistical significance (p = 0.056).