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Table 1 Real-time PCR primer sequences

From: A multi-target antisense approach against PDE4 and PDE7 reduces smoke-induced lung inflammation in mice

Primer

Sequence 5'-3'

Annealing Temperature (°C)

huPDE4B for

tggcagacctgaagacaatg

59

huPDE4B rev.1

aaattcctccatgatgcgg  

 

huPDE4B rev.2a

tctttgtctccctgctgga   

 

huPDE4D for

cagaatatggtgcactgtgc 

59

huPDE4D rev

agtctatgaagcccacctgtg

 

huPDE7A for

tcaggccatgcactgttact 

57

huPDE7A rev

cctgattctctcaataagccc

 

huPbib for

agagcatctacggtgagcg 

57

huPbib rev

cttccgcaccacctcca   

 
  1. aReverse primer used for first strand reaction
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Respiratory Research

ISSN: 1465-993X

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