Figure 5

Target mRNA expression in BAL cells following treatment with 4B/4D and 7A AON and smoke exposure. Mice were treated with combined 4B/4D and 7A AON (0.008, 0.04 or 0.2 mg/kg/day) or with control sequences (CTRL, 0.2 mg/kg/day) and exposed to cigarette smoke for one week, as per protocol design described in Figure 3A. PDE4B (A), PDE4D (B) and PDE7A (C) mRNA expression were quantified in BAL cells lysates using the Quantigene^® assay, and normalized to the expression of GAPDH used as a reference gene. Results are expressed as percentage of PDE mRNA levels in vehicle-treated and smoke-exposed animals (± SEM). *: p < 0.05; **: p < 0.01; ***: p < 0.001; t-test versus CTRL (compilation of 4 independent experiments, n = 10 to 20 mice per group).