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Figure 2 | Respiratory Research

Figure 2

From: A multi-target antisense approach against PDE4 and PDE7 reduces smoke-induced lung inflammation in mice

Figure 2

Inhibition of inflammatory mediators by combined 4B/4D and 7A AONs in NHBE cells. NHBE cells were transfected for 24 h with combined 4B/4D and 7A AON or with control sequences (CTRL) at 134 or 267 nM each, or were not transfected (NT). 4 h before the end of the transfection period, fresh media containing TNF-α, IL-1β and IFN-γ was added. Non-stimulated, non-transfected cells are shown as NS-NT. IL-8 (A) and MCP-1 (B) proteins were measured in cell culture supernatants by ELISA. Results are expressed as percentage of chemokine levels in non-transfected cells (± SEM). **: p < 0.01; ***: p < 0.001; t-test versus NS-NT or versus CTRL, as indicated (n = 6 replicates).

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Respiratory Research

ISSN: 1465-993X

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