Figure 7
From: Macrophage CD74 contributes to MIF-induced pulmonary inflammation
Anti-CD74 antibody treatment inhibits MIF-induced neutrophil accumulation into the alveolar space. A. The number of neutrophils in BALF from treated mice. The groups of mice were instilled via the trachea with either 1.0 μg MIF plus 10 μg anti-CD74 antibody or 1.0 μg MIF plus 10 μg control IgG. The other groups of mice were instilled with either 1.0 μg MIF plus 0.5 mg ISO-1 or 1.0 μg MIF plus 5% DMSO. Mice were euthanized 3 hours post instillation, then lungs were lavaged three times with normal saline. The number of neutrophils were counted using a hemocytometer. *P < 0.05 compared with control IgG + MIF or DMSO + MIF stimulation, B. MIP-2, and C. KC concentration in BAL fluid from 3 hours post instilled mice measured by ELISA. Bars indicate mean ± SD (n = 6/group). *P < 0.05 compared with control IgG + MIF or DMSO + MIF stimulation.