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Figure 6 | Respiratory Research

Figure 6

From: Macrophage CD74 contributes to MIF-induced pulmonary inflammation

Figure 6

Anti-CD74 antibody treatment inhibits MIF-induced p44/p42 MAPK phosphorylation and MIP-2 accumulation in macrophages. A. RAW264.7 cells were treated with 10 μg/ml anti-CD74 antibody or control IgG, or 100 mM ISO-1 or DMSO control for 10 min. Cells were lysed and subjected to SDS-PAGE, immunoprobed by anti-phospho and total p44/p42 MAPK antibody. Bar graphs show the density ratio of phospho- vs. total-p44/p42 MAPK bands. Bar graphs represent the mean ± SD of three independent experiments. B. RAW264.7 cells were treated with 10 μg/ml anti-CD74 antibody or control IgG, or 100 mM ISO-1 or DMSO control for 18 hours. After 18 hours supernatant were collected and MIP-2 concentration were measured by ELISA. Bars indicate mean ± SD (n = 3/group). Data represents four independent experiments. *P < 0.05 compared with control IgG + MIF or DMSO + MIF stimulation.

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