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Figure 3 | Respiratory Research

Figure 3

From: Macrophage CD74 contributes to MIF-induced pulmonary inflammation

Figure 3

CD74 expresses on the cell surface of alveolar macrophages. A. RAW264.7, MLE-12 and lung tissues from two non-treated mice were homogenized in lysis buffer and prepared for western blot analysis for CD74. The blots were then stripped and re-probed for GAPDH antibody as a loading control (total protein content was 15 μg/lane). Data represents three independent experiments. B. BAL cells from mice were stained with anti-CD74 antibody or control IgG and subsequent FITC-conjugated secondary antibody after fixation and permeabilization. (i), (ii) show the control IgG and (iii), (iv) show anti-CD74 antibody. (i), (iii) show differential interference contrast (DIC) and (ii), (iv) show fluorescent image. Green color represents CD74 or control IgG (FITC) and blue color represents nuclear (DAPI). Scale bar represents 20 μm. Data represents four independent experiments. C. Flow cytometry of non-permeabilized RAW264.7, MLE-12 and alveolar macrophages assessing surface expression of CD74. Shaded histograms show CD74 staining, and opened histograms show the control IgG staining. Data shown is representative of three independent experiments.

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