Table 1 Sequences of oligonucleotides used as forward and reverse primers for real-time RT-PCR.
Gene Product | Forward Primer | Reverse Primer |
|---|---|---|
Amphiregulin | 5'-TTTCGCTGGCGCTCTCA-3' | 5'-TTCCAACCCAGCTGCATAATG-3' |
ANP | 5'-CCAGGCCATATTGGAGCAAA-3' | 5'-AGGTTCTTGAAATCCATCAGATCTG-3' |
BNP | 5'-GAAGCTGCTGGAGCTGATAAGAG-3' | 5'-TGTAGGGCCTTGGTCCTTTG-3' |
CNP | 5'-AGGCAGCTGGTGGCAATC-3' | 5'-GCGATCGGTCTCCCTTGAG-3' |
FGFR4 | 5'-GTTGGCACGCAGCTCCTT-3' | 5'-GCAGGACCTTGTCCAGAGCTT-3' |
IL-6 | 5'-ATATGTTCTCAGGGAGATCTTGGAA-3' | 5'-TGCATCATCGCTGTTCATACAA-3' |
NPR-A | 5'-CCTCCTGACGTCCCTAAATGTG-3' | 5'-CCAGTGTGGAAAAGTGGTCTTG-3' |
NPR-B | 5'-TGAGCAAGCCACCCACTTC-3' | 5'-CAGCGGGCCGCAGATATA-3' |
NPR-C | 5'-ACCAACAGCTCTCCTTGCAAA-3' | 5'-AGGGCCCCCACAACAATT-3' |
PAI-1 | 5'-AGCTGGGCATGACTGACATCT-3' | 5'-GCTGCTCTTGGTCGGAAAGA-3' |
TF | 5'-CCCAGAAAGCATCACCAAGTG-3' | 5'-TGCTCCACAATGATGAGTGTT-3' |
VEGFR2 | 5'-CCACCCCAGAAATGTACCAAAC-3' | 5'-AAAACGCGGGTCTCTGGTT-3' |
β-actin | 5'-TTCAACACCCCAGCCATGT-3' | 5'-AGTGGTACGACCAGAGGCATACA-3' |